![]() This method is used for analysis of DNA sequences. Southern blotting is named after Edward M. Examples: Ethidium bromide, Crystal violet, Safranine and Ossmium tetroxide etc. And then we can visualize these transferring molecules by using staining. But sometimes it can be done by directly transferring the molecules onto the membrane. This process can be done just after the gel electrophoresis, by transferring the molecules from the gel onto the surface of blotting membrane. ![]() This membrane may be nitrocellulose PVDF or nylon membrane. Southern, Northern, Western Blotting, Probe, Hybridization, Antibody, Membrane Introductionīlotting is technique in which nucleic acids i.e., RNA and DNA or proteins are transferred onto a specific membrane. Finally, by using probe we have to detect the molecule of interest. Each technique depends upon the following factors such as the size of molecule and their binding ability to the solid support. ![]() These methods such as southern, western, northern and eastern are applicable for different types of macromolecules like lipids, RNA, DNA and proteins. Blotting is a common technique which is widely used in the field of molecular biology. ![]()
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